Kinetic and Structural Analysis of Substrate Specificity in Two Copper Amine Oxidases from Hansenula polymorpha

نویسندگان
چکیده

برای دانلود باید عضویت طلایی داشته باشید

برای دانلود متن کامل این مقاله و بیش از 32 میلیون مقاله دیگر ابتدا ثبت نام کنید

اگر عضو سایت هستید لطفا وارد حساب کاربری خود شوید

منابع مشابه

Kinetic and structural analysis of substrate specificity in two copper amine oxidases from Hansenula polymorpha.

The structural underpinnings of enzyme substrate specificity are investigated in a pair of copper amine oxidases (CAOs) from Hansenula polymorpha (HPAO-1 and HPAO-2). The X-ray crystal structure (to 2.0 A resolution) and steady state kinetic data of the second copper amine oxidase (HPAO-2) are presented for comparison to those of HPAO-1. Despite 34% sequence identity and superimposable active s...

متن کامل

Substrate specificity of amine oxidases.

DuBois, K. P., Albaum, H. G. & Potter, V. R. (1943). J. biol. Chem. 147, 699. Green, H. N. (1943). Lancet, 2, 147. Green, H. N. & Stoner, H. B. (1944). Brit. J. exp. Path. 25, 150. Kal¢kar, H. M. (1943). J. biol. Chem. 148, 127. Kolthoff, I. M. (1932). Saure-Ba8en Indicatoren. Berlin: Springer. Krebs, H. A. & Henseleit, K. (1932). Ho.ppe-Seyl. Z. 210, 33.' Lyubimova, M. N. & Pevsner, D. (1941)....

متن کامل

Inhibition of chickpea seedling copper amine oxidases by tetraethylenepentamine

Copper amine oxidases are important enzymes, which contribute to the regulation of mono- and polyamine levels. Each monomer contains one Cu(II) ion and 2,4,5-trihydroxyphenylalanine (TPQ) as cofactors. They catalyze the oxidative deamination of primary amines to aldehydes with a ping-pong mechanism consisting of a transamination. The mechanism is followed by the transfer of two electrons to mol...

متن کامل

The Substrate Specificity and Inhibition Char- Acteristics of Two Copper-protein “oxidases””

Evidence has been presented in earlier publications that the rapid oxidation of ascorbic acid in plant press-juices, when exposed to air, is primarily due to the catalytic effect of copper combined with protein. The evidence was of three types : (a) sensitivity to a number of organic and inorganic copper inhibitors, (b) the presence of adequate quantities of copper in the press-juices and enzym...

متن کامل

Pexophagy in Hansenula polymorpha.

In the yeast Hansenula polymorpha the development and turnover of peroxisomes is readily achieved by manipulation of the cultivation conditions. The organelles massively develop when the cells are incubated in the presence of methanol as the sole source of carbon and energy. However, they are rapidly and selectively degraded when methanol-grown cells are placed at conditions of repression of me...

متن کامل

ذخیره در منابع من


  با ذخیره ی این منبع در منابع من، دسترسی به آن را برای استفاده های بعدی آسان تر کنید

ژورنال

عنوان ژورنال: Biochemistry

سال: 2010

ISSN: 0006-2960,1520-4995

DOI: 10.1021/bi901933d